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Rf values and applications

Chromatography and Separation Techniques · Chemistry

Molecule viewer

Interactive 3D

Ball-and-stick models with true bond angles — switch molecule to compare shapes.

Controls

Drag the sliders or type a value — the simulation updates live.

Study notes

An unknown and standard both show Rf 0.45 in the same TLC run. Same compound? Step 1: Same plate, same solvent, same conditions → comparable. Step 2: Identical Rf suggests same compound — but not proof alone. Step 3: Co-TLC: spot mixture — one spot = same; two spots = different. Step 4: Rf + co-spotting = confident ID!

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